C (3-6 months) Bcl6遺伝子のfloxedマウス。エクソン7から9がloxPで挟まれている。Mb1-creマウスおよび、IgG1-creマウスとの交配により、B細胞免疫応答を検討したところ、大きな変化は見られていない。ただし、高親和性抗体は産生されてこない。 Bcl6-Flox mouse, B6-Bcl6 floxed Bcl6-Flox mouse, B6-Bcl6 floxed <A HREF="https://mus.brc.riken.jp/en/mouse_of_month/jun_2013_mm" target="_blank">Mouse of the Month Jun 2013</A> Developed by Toshitada Takemori, RIKEN Center for Allergy and Immunology in 2008. Bruce 4 ES cells derived from B6.Cg-Thy1.1 were used. A frt flanked neo cassette was removed by crossing with CAG-FLPe mice#23 (B6). Homozygote x Homozygote [or Crossing to C57BL/6JJcl] Homozygote x Homozygote [or Crossing to C57BL/6JJcl] RBRC05663 FLP/frt system Bruce 4 [B6.Cg-Thy1<a>] B6.Cg-Bcl6<tm1.1Mtto> Immunology and Inflammation Research <a href='https://brc.riken.jp/mus/pcr05663'>Genotyping protocol -PCR-</a> 理化学研究所免疫・アレルギー科学総合研究センター・竹森利忠先生 (2008)。Bruce 4 ES細胞を用いて作製。CAG-FLPeマウス (ライン36) との交配により、FRTで挟まれたneo遺伝子は除去済み。 Bcl6 gene floxed mice. Exons 7 to 9 were flanked by loxP sites. Phage P1 loxP sites, yeast FRT (flipase recombination target) site, mouse Bcl6 genome DNA Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested. Distinct cellular pathways select germ-line encoded and somatically mutated antibodies into immunological memory. J. Exp. Med., 209, 2079-2097 (2012). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Necessary documents for ordering:<ol><li>Approval form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_6.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_d.docx">English</A>)</li><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol> C（3〜6か月） B6.Cg-Bcl6<tm1.1> 条件を付加する。利用者は提供承諾書を用いて、事前に寄託者の承諾を得る。<br>利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する。Distinct cellular pathways select germ-line encoded and somatically mutated antibodies into immunological memory. J. Exp. Med., 209, 2079-2097 (2012). 利用者は、研究成果の公表にあたって謝辞の表明を必要とする。 true 竹森　利忠 Cre/loxP system Toshitada TAKEMORI